Structural and mechanistic aspects of flavoproteins

Structural and mechanistic aspects of Amt/Rh proteins.

Amt/Rh proteins, which mediate movement of ammonium across cell membranes, are spread throughout the three kingdoms of life. Most functional studies on various members of the family have been performed using cellular assays in heterologous expression systems, which are, however, not very well suited for detailed mechanistic studies. Although now generally considered to be ammonia conducting cha...

Kinetic, Mechanistic, and Structural Aspects of Unliganded Gating of Acetylcholine Receptor Channels

The spontaneous activity of adult mouse muscle acetylcholine receptor channels, transiently expressed in HEK-293 cells, was studied with the patch-clamp technique. To increase the frequency of unliganded openings, mutations at the 12' position of the second transmembrane segment were engineered. Our results indicate that: (a) in both wild type and mutants, a C <--> O kinetic scheme provides a g...

Structural Elucidation of the Mechanistic

Oxidation by permanganate: synthetic and mechanistic aspects

Oxidation by permanganate: synthetic and mechanistic aspects Sukalyan Dash , Sabita Patel , Bijay K. Mishra a,* Centre of Studies in Surface Science and Technology, Department of Chemistry, Sambalpur University, Jyoti Vihar, Burla 768 019, India Department of Chemistry, University College of Engineering, Burla 768 018, India Department of Chemistry, National Institute of Technology, Rourkela 76...

Enzyme promiscuity: evolutionary and mechanistic aspects.

The past few years have seen significant advances in research related to the 'latent skills' of enzymes - namely, their capacity to promiscuously catalyze reactions other than the ones they evolved for. These advances regard (i) the mechanism of catalytic promiscuity - how enzymes, that generally exert exquisite specificity, promiscuously catalyze other, and sometimes barely related, reactions;...